Detection of Novel gyrB Mutation in Fluoroquinolone-Resistant Salmonella and Escherichia coli using PCR-RFLP
نویسندگان
چکیده
Abstract Background Emergence of fluoroquinolone resistance in gut pathogens is a cause concern. Resistant to quinolone mainly due the point mutations at quinolone-resistance determining regions (QRDR). The aim study was develop polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP) detect QRDR gyrA and gyrB enteric pathogens. Methodology PCR-RFLP done for 83 region using HinfI 447 AcuI resistant susceptible products were also sequenced confirm presence restriction sites. Results In this study, technique developed Salmonella typhi Escherichia coli. A first its kind mutation enzyme AcuI. Restriction digestion resulted three bands S. isolates four seen sensitive isolates, while two E. coli isolates. Similarly, no This suggest that there ofE. coli, found Conclusion present could successfully fluoroquinolone-resistant can be efficiently used epidemiological studies instead cost-intensive sequencing method status multiple
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ژورنال
عنوان ژورنال: Journal of Health and Allied Sciences NU
سال: 2022
ISSN: ['2582-4953', '2582-4287']
DOI: https://doi.org/10.1055/s-0042-1755597